In our study, we found that combined with cerulein treatment, mice injected with anti-Shh antibody developed more severe acute pancreatitis than the mice in the IgG and PBS control groups, as the MPO activity, serum lipase and amylase levels in mice blood and tissue evaluation by HE staining from anti-Shh antibody treated group were all significantly severe or higher compared with the control ones. The gene discussed is SHH; the disease is acute pancreatitis.