We found that level of TNF-α and ET-1 was higher in 12mL/kg and sepsis induced ALI groups, and These mean that 6mL/kg tidal volume could decrease cyclic stretch(alveolar tidal opening-closing and hyperinflation), inflammatory response and protect endothelium-dependent relaxation from LPS-induced lung injury. Here, EDN1 is linked to acute respiratory distress syndrome.