One gene, FZD1, which was expressed at lower levels in CAFs derived from Her2+ breast cancer by array analyses, was also significantly lower by qRT-PCR in the Her2/TNBC comparison but was not significantly different in the ER/TNBC comparison (P = 0.2) although fold change values were similar by qRT-PCR (TNBC/ER + = 1.33 for microarrays and 1.39 for qPCR). This evidence concerns the gene ERBB2 and breast cancer.