Thus, we selected 10 genes (PPEF2, PRDM8, SDAD1, CXCL9, CCDC158, PRKG2, HPSE, AGPAT9, HELQ, NUP54) with the highest frequency of Gene LOH (Table S2) and another gene (CCNG2) based literature reports, which suggested CCNG2 as a tumor suppressor, to detect mRNA expression level in 50 pairs of HCC and the matched liver samples from the 112 cases. Here, PPEF2 is linked to hepatocellular carcinoma.