MCAM and neoplasm: In contrast, we found that reduced CD146 expression in MDA-MB-231 and CAL51 cell lines inhibited anchorage-independent growth rate, while over-expression of CD146 in SKBR3 cells increased its anchorage-independent growth ability (Fig. 3B), suggesting that CD146 contributes to tumor transformation in several breast carcinoma cell lines except MCF-7.