We assessed the expression of HIF-1α in PDAC samples by immunohistochemistry and found that HIF-1α was correlated with tumor diameter (F = 7.81, P<0.01), lymph node metastasis (r = 0.24, P<0.05), the pathological tumor node metastasis stage (r = 0.39, P<0.01) and PNI (r = 0.43, P<0.01) of PDAC samples (Table 1). The gene discussed is HIF1A; the disease is neoplasm.