WASF3 and breast cancer: WAVE3 expression levels were assessed by western blotting in a series of six BC cell lines and was found to be highly expressed in MDA-MB-231, BT549 and MDA-MB-435s, all of TN basal subtype, while WAVE3 expression levels were comparatively very low in MCF7, T47D and SKBr3 BC cell lines of luminal subtype (Fig. 1A).