Although all of these variations were found in genes previously known to be associated with hearing loss, 5 of the 7 variations were novel missense (p.D187H in ACTG1, p.P678S in DIAPH1, p.E232K in POU4F3 and p.P1422L in COL11A2) or nonsense mutations (p.S288X in EYA4) that had not been recorded in any public databases[12-18]. The gene discussed is EYA4; the disease is hearing loss disorder.