To determine whether TLR4 is important in LPS-induced tumor cell metastasis and EMT, we used adeno-associated virus to express TLR4 in HepG2 cells [See, Additional file 1, Figure S2A, B], then pretreated vec-HepG2 and TLR4-HepG2 with LPS. This evidence concerns the gene TLR4 and neoplasm.