Here we report the analysis of activating mutations in codon 816 of c-KIT in 31 patients with a GB and/or dysgerminoma by LightCycler analysis, together with conventional sequence analysis of c-KIT exons 8, 9, 11, 13 and 17, and PDGFRA exons 12, 14 and 18, mutations in which are frequently found in GIST. This evidence concerns the gene KIT and dysgerminoma.