The in vivo effects of compound 4EGI-1 on the formation of eIF4F complex were investigated by pulling-down eIF4E from tumor lysates by 7-methylguanosine diphosphate (M7GDP)-Sepharose affinity chromatography followed by WB analysis of eIF4E, eIF4G and 4E-BP1. Here, EIF4EBP1 is linked to neoplasm.