In the current study, the effect of NDV on cancer cells in the absence of Bcl-2 and Bax was investigated; HT29 human colon adenocarcinoma cell line (originally Bcl-2-free, purchased from ATCC), HCT116 Bax−/− (kindly provided by Bert Vogelstein, Johns Hopkins Medical University, MD, USA) and wt colorectal carcinoma cell lines (kindly provided by Eric Stanbridge, University of California, Irvine, CA, USA) were cultured in 6-well plates and infected with NDV strain AF2240 according to a standard protocol (30 HA units of NDV for 1.0 × 106 cultured cells)[21]. The gene discussed is BAX; the disease is colon adenocarcinoma.