Three days post-seeding into the wells, the migratory forefronts of non-targeting shRNA endothelial cells and the melanoma cells had collided, while the forefronts of ROCK1 or 2 knockdown cells were 670 +/- 80 and 920 +/- 110 μm apart, respectively (Fig. 3B, D). This evidence concerns the gene ROCK1 and melanoma.