Similarly, the expression of the gene fusion product KIAA1549/BRAF in juvenile pilocytic astrocytoma (JPA) (Jones et al., 2009) may be detectable in a similar manner, since another form of mutant BRAF (V600E) was found in EVs collected from plasma of melanoma patients (Ramachandran et al., 2011). This evidence concerns the gene BRAF and melanoma.