EGFR and squamous cell carcinoma: In A431 squamous cell carcinoma cells harboring an amplified EGFR gene, stimulation with EGFR ligands (TGFα) coupled with blockade of E-cadherin results in an EMT-like state characterized by the onset of vimentin expression, and spindle morphology, as well as internalization of cell surface receptors, and a profoundly altered vesiculation profile.