Figure 4D shows the log2-transformed fold changes of BRMS1 and OPN mRNA expression ratio of T/N (Tumor/Non-tumorous tissue). While BRMS1 expression exhibited a consistent down-regulated pattern, OPN expression was significantly up-regulated in HCC tissues compared to non-tumorous tissues. More importantly, a negative correlation (P = 0.021, Fisher's exact test) between BRMS1 and OPN mRNA expression was identified (Table 1). The gene discussed is SPP1; the disease is hepatocellular carcinoma.