We also investigated the role of I148M PNPLA3 variant on NAFLD severity and, according to previous report [19], we found that subjects homozygous for I148 PNPLA3 variant were completely protected from NASH, whereas all M148 PNPLA3 homozygous patients showed definitive NASH. This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatohepatitis.