The T6SS loci of V. cholerae (the causative agent of cholera) consist of the 17-gene vas cluster (VCA0107-0123), the hcp1-vgrG1 operon (VC1415–VC1416), and the hcp2-vgrG2 operon (VCA0017–0018) [24]. Here, CYCSP52 is linked to vibrio infectious disease.