We then performed the MTT assay to investigate the effects of EGF androgen and antiandrogen on cell proliferation of bladder cancer lines with vs. without AR (i.e., UMUC3-control-shRNA vs. UMUC3-AR-shRNA, 5637-AR vs. 5637-V and J82-AR vs. J82-V). Here, EGF is linked to urinary bladder cancer.