Another breakpoint was observed at NAALAD2 gene in KYSE510, and it was located within FRA11F. In addition, breakpoints proximal of 11q13 amplicons in KYSE180, KYSE510 and five online cell lines were located in FRA11A region in 11q13.3, while the proximal breakpoints in KYSE30 and other online cell lines were distal to FRA11A or in FRA11H. In the tested ESCC tumors, the majority of breakpoints in 11q13.3(1) were proximal to the amplicons, and most of those in 11q13.3(2) and 11q13.4 were distal to the amplicons. This evidence concerns the gene NAALAD2 and esophageal squamous cell carcinoma.