In PPROM <34.0 weeks, high intra-amniotic inflammatory response, mediated by IL-6, IL-8, IL-10, BDNF, GMC-SF, MCP-1, MIP-1, MMP-9 and TREM-1, was observed when both MIAC and HCA were present. The gene discussed is BDNF; the disease is preterm premature rupture of the membranes.