In this study, we have developed conditional deletion of exon 3–6 of the Lkb1 gene in the Müllerian duct mesenchyme-derived stromal cells of the murine female reproductive tract by crossing Müllerian inhibiting substance type 2 receptor-drive Cre (Misr2-Cre, also known as antiMüllerian hormone type 2 receptor Amhr2-Cre) [16] mice with Lkb1fl/fl[19] mice to develop Lkb1cko mice to determine whether the loss of LKB1 affects either female reproductive development or results in PJS-associated pathologies. Here, STK11 is linked to Peutz-Jeghers syndrome.