To test the role of Ccr1 in neutrophil trafficking from the blood to the kidney directly, we conducted a competitive repopulation study where equal numbers of Ccr1+/+ and Ccr1−/− Ly6cintLy6G+CD11b+ neutrophils were isolated by gradient centrifugation from the bone marrows of Ccr1+/+ and Ccr1−/− Candida-infected mice at day 9 post-infection, then differentially labeled, mixed 1∶1 and injected into Candida-infected Ccr1+/+ recipient mice on day 9 post infection. Here, CCR1 is linked to infection.