To examine if C. glabrata cells encounter oxidative stress-induced DNA damage upon macrophage ingestion, we quantified the phosphorylation of H2AX (γ-H2AX, double-strand break-induced serine-129 phosphorylated form of H2A variant) and found it to be significantly increased in macrophage-internalized wt cells 2 h post infection (Figures 2B and S4A and C). This evidence concerns the gene H2AX and infection.