PBMCs cultured with both blocking antibodies (αPD-L1 and αHVEM) also significantly enhanced T cell proliferation however, the effect was not synergistic compared to using αHVEM alone (P<0.0001) (Figure 4B,C).We analyzed the coexpression of BTLA and CD160 on HIV-specific CD8 T cells during chronic HIV infection and found that the frequency of CD160 (35.5%) was significantly greater than BTLA (3.9%; P<0.0001) suggesting that using αHVEM preferentially disrupts the CD160/HVEM axis (Figure S8). The gene discussed is CD8A; the disease is HIV infectious disease.