Since the mdx mouse originates from a spontaneous mutation in exon 23 of the dystrophin gene and was previously demonstrated to determine muscle pathophysiology of DMD [18], [19], [20], we decided to investigated the role of miRNAs in muscle remodelling by evaluating the miRNome of single muscle fibres isolated from three different muscles (Tibialis Anterior, TA; Diaphragm, DIA and Vastus, VA) of this animal model. Here, DMD is linked to Duchenne muscular dystrophy.