We further analyzed expression of Ano1 in three different HNSCC cell lines: CAL-27 and CAL-33 (two tongue squamous cell carcinoma cell lines without genomic amplification of Ano1) and BHY cells (an oral squamous cell carcinoma cell line with known 11q13-amplification [25], [26]. Here, ANO1 is linked to head and neck squamous cell carcinoma.