Human LAGE3 belongs to the NY-ESO gene family together with the closely related LAGE1 and LAGE2 [24], and all three genes are clustered in the same region on chromosome X. While LAGE3 is ubiquitously expressed, LAGE1 and LAGE2 are cancer-testis antigens with high expression in healthy testis and upregulation in a number of cancer tissues, similarly to PRAME. Here, PRAME is linked to cancer.