The age of the founder event was estimated to approximately 700 years for c.1293insG mutation in CHRNE gene [45], 80 generations for the autosomal non syndromic optic atrophy (2400 years) [43] and calculated to have arisen in the second half of the 13th century for Creutzefeldt-Jacob disease [87]. This evidence concerns the gene CHRNE and Leber hereditary optic neuropathy.