Compared to the background seen with NY-ESO-1−/HLA-A2+ melanoma cell lines SK-Mel-29-VC (196 pg/ml) and Me-324 (115 pg/ml) (Fig. 2d), the concentration of released IFN-γ was higher for KT-195-A2 cells, probably due to the overexpression of HLA-A2 on these cells. This evidence concerns the gene IFNG and melanoma.