APP and Alzheimer disease: To identify known pathogenic mutations and novel rare variants, pooled-DNA sequencing was performed for the coding exons and their corresponding flanking regions for APP, PSEN1, PSEN2, MAPT, and GRN in a total of 15 (8.98%) familial EOAD, 136 (81.44%) sporadic EOAD, 16 (9.58%) familial LOAD, and five autopsy-confirmed AD cases (Table 1).