In order to determine whether the expression of Hif-2αinfluenced the expression of Klf5 in glioma cells, we transduced Rt-glioma cells with an inducibly-expressed variant of Hif-2α(Hif-2αP531A) that is degradation-resistant under normoxia (Figure 3A) [37]. The gene discussed is EPAS1; the disease is glioma.