To probe possible interactions between bacterial inclusions and the host ER, HeLa cells were initially fixed 24 hours post-infection (hpi) with Chlamydia trachomatis LGV2, and mid-stage inclusions containing replicative chlamydial RBs (1) examined in relation to the ER marker calreticulin (16) by confocal microscopy. The gene discussed is CALR; the disease is infection.