SMOX and cystic fibrosis: Since the growth of PAO1 in ASM+ appears similar to the macrocolonies reported within the lungs of CF patients with chronic P. aeruginosa infection[21], we inoculated ASM+ with PAO1/pMRP9-1 as described above and incubated the cultures in 20% EO2 at 37°C for up to 16 d.