In addition, primary tumor weights were significantly less in the Gal3 knock down group than the control group as demonstrated in Figure 4 E; Immunohistochemical staining for Gal-3, Ras and phospho-ERK in these mice tumor tissues (Figure 4F) further confirmed that expression of Gal-3, Ras and the down-stream effector phospho-ERK are decreased in the Gal-3 knock down group compared with the control group. This evidence concerns the gene LGALS3 and neoplasm.