In order to determine if Gal-3 mRNA was also up-regulated in mouse tumor tissues compared with normal and pancreatitis tissues, tissues from individual mice that were normal, had pancreatitis or those with tumors were extracted and real-time PCR was performed using specific mouse Gal-3 primers (Mm00802901_m1, Ambion, USA) (Figure 2B). The gene discussed is LGALS3; the disease is pancreatitis.