LGALS3 and pancreatic neoplasm: In order to determine if Gal-3 plays a functional role in pancreatic tumor cell behavior in vitro, we utilized cell lines genetically altered to express different Gal-3 levels as shown in Figure 3A to determine the effects of Gal-3 on tumor cell growth (MTS assay), invasion (Matrigel invasion assay) and in vitro tumorigenicity (soft agar colony formation assay).