At 10 h post infection, L/GFP-LC3 cells contained few cytoplasmic GFP-LC3 puncta with 5.4% of infected cells having co-localization with mRFP-VP26 (Fig. 6A, arrowheads), however by 24 h post infection co-localization of the two markers was apparent in 1.7% of infected L/GFP-LC3 cells. Here, MAP1LC3A is linked to infection.