HE, MGT, and ATPase stains are essential for the diagnosis and differential diagnosis of muscle diseases; PAS, oil red O, NADH-TR, SDH, COX, myophosphorylase, amylopectinase phosphofructokinase, and adenylate deaminase staining were used to exclude glycogen storage diseases, mitochondrial myopathy, lipid storage myopathy, and so on. This evidence concerns the gene PYGM and Glycogen storage disease due to glycogenin deficiency.