HE, MGT, and ATPase stains are essential for the diagnosis and differential diagnosis of muscle diseases; PAS, oil red O, NADH-TR, SDH, COX, myophosphorylase, amylopectinase phosphofructokinase, and adenylate deaminase staining were used to exclude glycogen storage diseases, mitochondrial myopathy, lipid storage myopathy, and so on. Here, PYGM is linked to myopathy with abnormal lipid metabolism.