Fourty-three of the 111 PBC patients were ANA positive, 16 with MND pattern, 5 MND and peri-nuclear, 4 peri-nuclear, 1 peri-nuclear and anti-nucleolar, 12 speckled, 1 speckled and MND, 1 speckled and anti-nucleolar and 3 diffuse. This evidence concerns the gene BTG3 and primary biliary cholangitis.