Quezada et al. [57] showed effector CD4+ T-cell-dependent tumor rejection when radiotherapy and adoptive transfer of tumor-reactive CD4+ T cells were combined with the blockade of the CTLA-4 molecule that is expressed on Tregs [60, 61], suggesting that Tregs influenced cytotoxic CD4+ T cells in this model as well. The gene discussed is CTLA4; the disease is neoplasm.