As the human MD-2 amino acid residues binding LPS and the proposed binding sites for PAMAM-DG (Barata et al, 2011b) in small animals are most conserved in rabbit (Human/rabbit MD-2: K91K;R96K;S98S;Y102Y;R106R;K109K;T112T;N114N;T116T;S118P), a rabbit intestinal loop model of shigellosis was used to determine the bioactivity of PAMAM-DG (Perdomo et al, 1994; Raqib et al, 1995; Singer & Sansonetti, 2004). The gene discussed is LY96; the disease is shigellosis.