In the current study, the effect of FASN inhibition in the cellular uptake of the metabolic precursors of fatty acids was investigated for the first time in NSCLC cell lines with different molecular and genetic abnormalities that affect FASN expression activity levels by comparing the in vitro accumulation of [3H]acetate, [14C]fluoroacetate (as a surrogate of [18F]fluoroacetate), and [18F]FDG. Here, FASN is linked to non-small cell lung carcinoma.