AKT1 and acute lymphoblastic leukemia: To determine whether treatment of T-ALL cell lines with inhibitors of PI3K/Akt/mTOR signaling could affect cell cycle progression, MOLT-4 cells were incubated for 24 h with increasing concentrations of the drugs (0.1-1 μM) and the cell cycle was studied by means of flow cytometric analysis of propidium iodide (PI) -stained samples.