To study the mechanism and functional role of the fascin-1/LIMK interaction in carcinoma cells, we first measured interactions of non-activatable (T508A) or catalytically inactive (D460A) forms of GFP-LIMK1 with mRFP-fascin-1 by FRET/FLIM in SW480 cells migrating on laminin. Here, LAMB2 is linked to carcinoma.