CCNB1 and cervical carcinoma: Fig. 1C showed that the expression of Erbin with a distinct molecular weight of 180 kDa varied during mitosis, rising in S phase and peaking in G2/M phase. Notably, the expression pattern was apparently coincident with that of cyclin B1, a known mitotic substrate of the anaphase promoting complex/cyclosome. To further validate the data, human cervical carcinoma cell line HeLa, human kidney cell line 293T, and immortalized human hepatic cell lines LO2 and HL-7702 were also treated with nocodazole. Similar data were obtained in all cell lines tested (Fig. 1D).