CRTC1 and hepatocellular carcinoma: To ascertain whether TORC1 was involved in controlling nuclear tRNA export in mammals, FISH was used to monitor the cellular distribution of tRNALys in HeLa and rat hepatoma H4IIE cells incubated in DMEM without serum and containing the drug vehicle solution (see figure legend) with or without 100 nM rapamycin for 4 h (Figure 1A).