To understand the mechanism of SPARC in ovarian cancer proliferation, apoptosis and invasion, real-time quantitative RT-PCR was used to detect the different expressions of E-cadherin, β-catenin, alpha-catenin, Integrin β3, Integrin β1, ILK, FAK, P53, P21, Cyclin D1, PCNA, Bcl-2, Bax, u-PA, uPAR, PAI-1, MMP2, MMP9, TIMP1 and TIMP2 between SPARC shRNA infected S1 subclone cells and control shRNA infected S1 subclone cells. This evidence concerns the gene TP53 and ovarian cancer.