Furthermore, Il10−/− mice developed enhanced body weight loss early upon infection (Figure 1C), reaching the nadir between day 5 and 6 p.i., but recovered thereafter to B6 weight levels by day 14 p.i. To determine whether the increased body weight loss in Il10−/− mice was a consequence of excessive cytokine production, we neutralized TNF-α (Figure 1C), since neutralization of IFN-γ was reported to have no effect on the development of pathology in Il10−/− mice [14]. This evidence concerns the gene TNF and infection.