The top 50 genes selected were manually analyzed with the scientific literature in order to validate its connection with PE or even changes during pregnancy and we corroborate that several of them like FLT1, FLT4, VEGFA, PGF, TNF, FN1, F2 and NOS3 can be related to the main lines of research in preeclampsia pathogenesis hypothesis and specially angiogenesis[11-16]. This evidence concerns the gene NOS3 and preeclampsia.