SCGB3A1 and cancer: To verify the role of DNA methylation and the level of expression of the HIN-1 gene, fully methylated KK cancer cells were treated with the demethylating agent, 5-aza-2-dC at 0, 24, 48, 72, 96 hours, and the density of unmethylated band of HIN-1 gene was gradually reversed after 48 hoursdetected by MS-PCRe shown in Figure4A. After normalization, relative HIN-1 mRNA levels in KK cells significantly increased after treatment with 5-aza-2-dC when the duration extended (Figure4B), which is correlated with HIN-1 DNA methylation (Figure4A).