To determine whether leptospiral hemolysins stimulate inflammatory responses, THP-1 or J774A.1 cells were treated with each of the five rL-hemolysin proteins (rSph1, rSph2, rSph3, rHlpA and rTlyA) that were confirmed to be secreted by L. interrogans strain Lai during infection of host cells. This evidence concerns the gene RSPH3 and infection.