To determine whether leptospiral hemolysins stimulate inflammatory responses, THP-1 or J774A.1 cells were treated with each of the five rL-hemolysin proteins (rSph1, rSph2, rSph3, rHlpA and rTlyA) that were confirmed to be secreted by L. interrogans strain Lai during infection of host cells. Here, RSPH1 is linked to infection.