In spite of the lack of HIV neutralizing antibodies, additional studies on the fine specificity of the Env antibody response induced by Ad35-GRIN/ENV are also warranted following the recent findings that IgG binding antibody to a scaffolded HIV-1 gp120 V1V2 protein encompassing the gut homing marker α4β7 integrin binding site was identified in a post-hoc analysis as a correlate of risk for acquisition of HIV infection in RV144 [64]. Here, ITIH4 is linked to HIV infectious disease.